DocumentCode :
3412083
Title :
Microchip-based bioelectrocatalytic determination of organophosphorus compounds
Author :
Yong Duk Han ; Chi Yong Jeong ; Jun Hee Lee ; Yoon, H.C. ; Dae Sik Lee
Author_Institution :
Dept. of Mol. Sci. & Technol., Ajou Univ., Suwon, South Korea
fYear :
2011
fDate :
3-5 Aug. 2011
Firstpage :
1
Lastpage :
3
Abstract :
We have developed a new detection method of organophosphorus (OP) nerve agent using acetylcholine esterase (AchE) and choline oxidase (ChOx) bienzyme system. AchE is inhibited by OP pesticide. So OP can be detected by the inhibition mechanism. To fabricate the AchE/ChOx bienzyme modified biosensing system, poly-L-lysine (PLL) and aldehyde terminated paramagnetic microparticle (MP) were used for enzyme immobilization. By using PLL, glutaraldehyde and amine rich electrode surface, ChOx were immobilized on the single micropatterned electrode. AchE was immobilized on the MP surface via Schiffs base formation. The resulting enzyme modified MPs were moved and deposited on the signaling electrode using magnet under micro fluidic channel. To achieve whole OP detection procedures on the single microchip, microfluidic channel was employed to the microelectrode. Based on the bioelectrocatalytic reaction between AchE/ChOx bienzyme system and ferrocenyl electron shuttle, we successfully practiced the OP (e.g. diazinon) biosensing with the developed microchip.
Keywords :
bioMEMS; biochemistry; biosensors; catalysis; electrochemical electrodes; electrochemical sensors; enzymes; lab-on-a-chip; microelectrodes; microfluidics; microsensors; molecular biophysics; neurophysiology; AchE-ChOx bienzyme modified biosensing system; OP biosensing; OP pesticide; Schiff base formation; acetylcholine esterase; aldehyde terminated paramagnetic microparticle; amine rich electrode surface; bioelectrocatalytic reaction; choline oxidase bienzyme system; enzyme immobilization; ferrocenyl electron shuttle; inhibition mechanism; microchip-based bioelectrocatalytic determination; microelectrode; microfluidic channel; organophosphorus compounds; organophosphorus nerve; poly-L-lysine; signaling electrode; single micropatterned electrode; Biochemistry; Biosensors; Electrodes; Microfluidics; Silicon; Substrates; Surface treatment;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Defense Science Research Conference and Expo (DSR), 2011
Conference_Location :
Singapore
Print_ISBN :
978-1-4244-9276-3
Type :
conf
DOI :
10.1109/DSR.2011.6026832
Filename :
6026832
Link To Document :
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