Title :
Automated DNA sequencing and construction of consensus sequence
Author :
Danckaert, Anne ; Hazout, Serge ; Valleron, Alain-Jacques
Author_Institution :
INSERM, Paris, France
Abstract :
A novel image processing method called morphological automation, associated with an integrated device (gel swallowing), is proposed to automate the reading of DNA electrophoretic gels. It has been evaluated on the Mason-Pfizer-Monkey virus sequence. Up to 96.6% of the bases were found to be identical to the published sequence. Another algorithm permits reconstruction the entire DNA sequence.<>
Keywords :
DNA; biology computing; computerised pattern recognition; computerised picture processing; molecular biophysics; DNA construction; DNA sequencing; Mason-Pfizer-Monkey virus sequence; algorithm; consensus sequence; electrophoretic gels; gel swallowing; image processing method; morphological automation;
Conference_Titel :
Engineering in Medicine and Biology Society, 1988. Proceedings of the Annual International Conference of the IEEE
Conference_Location :
New Orleans, LA, USA
Print_ISBN :
0-7803-0785-2
DOI :
10.1109/IEMBS.1988.94560
