Genetic analysis on viable but non-culturable Salmonella pullorum by mRNA differential display

Salmonella pullorum was induced using liquid lysogeny broth medium at 4°C into viable but non-culturable (VBNC) state. The activated VBNC genes were screened by mRNA differential display. VBNC bacteria transformed to the spherical-rods or spherical shape and aggregated. Sequence analysis showed two VBNC differential fragments respectively belonged to partial sequences of ATP-dependent RNA unwindase rh1B gene and tRNA 2-selenouridine synthase ybbB gene in Salmonella pullorum. The former sequence may be the structure domain of ATPase responsible for coding DEAD-box unwindase, and the latter may catalyze 2-uridine converting into 2-selenium uridine in tRNA molecules, responsible for participation in the codon-anticodon interaction and the amino acylation of tRNA.