Rapid determination of lignans in Schisandra Chinensis and Schisandra sphenanthera by micellar electrokinetic capillary chromatography

A simple and rapid micellar electrokinetic capillary chromatography (MEKC) method has been developed for the simultaneous determination of six lignans, schizandrin, Gomisin A, schisantherin A, deoxyschizandrin, schizandrin B and schizandrin C, in fruits of S. chinensis and S. sphenanthera. The key factors for separation and determination were studied and the best analysis conditions were obtained, in the buffer of 10 mM phosphate-37.5 mM SDS-35% v/v acetonitrile (pH 8.0) at the separation voltage of 28.0 KV and detection at 214 nm, the plant samples could be detected within 8.2 min. Analysis yielded good reproducibility (RSD between 1.19-2.28%) and good recovery (between 92.2-102.9%). The method is quick, sensitive, accurate and it could be applied to the quality control of S. chinensis and S. sphenanthera.