Active positioning control of single cell/microbead in a micro-well array chip by dielectrophoresis

Author

Kim, Byoung-Gyun ; Yun, Kwang-Seok ; Yoon, Euisik

Author_Institution

Dept. of Electr. Eng. & Comput. Sci., Korea Adv. Inst. of Sci. & Technol., Daejeon, South Korea

fYear

2005

fDate

30 Jan.-3 Feb. 2005

Firstpage

702

Lastpage

705

Abstract

We present a micro-well array chip operated by negative dielectrophoresis for active positioning control of single cell or microbead. The prototype chip has been designed and fabricated to validate concept feasibility in a 2×2 micro-well array using silicon substrate and PDMS channel structures. We have successfully demonstrated capturing experiments of single polystyrene microbead (15μm) into each micro-well by dielectrophoresis with control electrical signals of 10V_pp at 1 MHz.

Keywords

biological techniques; cellular biophysics; electrophoresis; micromechanical devices; micropositioning; nanopositioning; 1 MHz; 10 V; 15 micron; PDMS channel structures; active positioning control; concept feasibility; dielectrophoresis; micro-well array chip; prototype chip; silicon substrate; single cell; single polystyrene microbead; Biochemical analysis; Cells (biology); Chip scale packaging; Dielectrophoresis; Electrodes; Frequency; Hydrodynamics; Microfluidics; Prototypes; Silicon;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on

ISSN

1084-6999

Print_ISBN

0-7803-8732-5

Type

conf

DOI

10.1109/MEMSYS.2005.1454026

Filename

1454026