Quantitative determination of total alkaloid in Corydalis Ochotensis Turcz by colorimetry

Author

Mao Meina ; Liu Yongqiang ; Zhou Mingjuan ; Chen Hongyan ; Zhao Chunfang

Author_Institution

Dept. of Pharmacy, Jilin Univ., Changchun, China

fYear

2011

fDate

19-22 Aug. 2011

Firstpage

32

Lastpage

34

Abstract

A quantitative determination of the total alkaloid in Corydalis Ochotensis Turcz was established by acid dye colorimetry. The effects of wavelength, pH of the phosphate buffer and the quantity of acid dye were studied. Two different dyes were compared according to the yield of berberine hydrochloride. The method with bromophenol blue dye was proved to be more accurate, more stable with good reproducibility in testing experiments. So, it was fit for quantitative determination of total alkaloid in Corydalis Ochotensis Turcz.

Keywords

biochemistry; chemical analysis; colorimetry; dyes; pH; acid dye colorimetry; berberine hydrochloride; bromophenol blue dye; corydalis ochotensis turcz; pH; phosphate buffer; quantitative determination; total alkaloid; Correlation; Educational institutions; Equations; Ethanol; Materials; Presses; Stability analysis; Acid dye colorimetry; Bromophenol blue; Corydalis Ochotensis Turcz; Total alkaloids;

fLanguage

English

Publisher

ieee

Conference_Titel

Human Health and Biomedical Engineering (HHBE), 2011 International Conference on

Conference_Location

Jilin

Print_ISBN

978-1-61284-723-8

Type

conf

DOI

10.1109/HHBE.2011.6027890

Filename

6027890