Fabrication of and signal recording from lipid membrane sensors

Author

Kuwana, Y. ; Tamada, Y.

Author_Institution

National Inst. of Agrobiological Sci., Tsukuba, Japan

fYear

2005

fDate

30 Jan.-3 Feb. 2005

Firstpage

826

Lastpage

829

Abstract

We are developing a new type of biosensor that combines insect receptor proteins with a field effect transistor (FET) as a transducer. Receptor proteins will be immobilized in a horizontal lipid bilayer membrane formed on the FET, and the FET will detect membrane potential changes caused by stimulus-receptor interactions. In the preliminary study reported here, two types of lipid membrane support system were fabricated. Optical microscopy and membrane capacitance measurements confirmed the formation of membranes. The possibility of real membrane potential measurement with this system was confirmed by measuring a simulated membrane potential.

Keywords

biomembranes; biosensors; field effect transistors; lipid bilayers; proteins; transducers; FET transducer; biosensor; field effect transistor; insect receptor proteins; lipid bilayer membrane; lipid membrane sensor; membrane capacitance measurement; membrane potential changes; optical microscopy; signal recording; simulated membrane potential; stimulus-receptor interaction; Biomedical optical imaging; Biomembranes; Biosensors; FETs; Fabrication; Insects; Lipidomics; Optical microscopy; Protein engineering; Transducers;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on

ISSN

1084-6999

Print_ISBN

0-7803-8732-5

Type

conf

DOI

10.1109/MEMSYS.2005.1454057

Filename

1454057