The role of AcSDKP on inhibiting myofibroblast differentiation in rats with silicosis

Myofibroblast differentiation, characterized by α-smooth muscle actin (α-SMA) expression, is a key process in organ fibrosis. Our recent studies have shown that AcSDKP has a anti-fibrotic effect in the lung of rats with silicosis. Here we explore the underlying effect of AcSDKP on myofibroblast differentiation during the anti-fibrotic process. SiO₂ powders were instilled in trachea of rat to make the silicotic model, and caused 1) increased expressions of TGF-β, 2) escalated serum response factor (SRF) expression, 3) myofibroblast marked by o-SMA being detected in siliconic nodules, and 4) collagen deposition in lung. AcSDKP (both anti fibronic and prevention treatment) can inhibit these changes induced by silica significantly. AcSDKP could inhibit collagen synthesis throuth blocking myofibroblast differentiation in the lung of rats with silicosis, which is a new mechanism of its anti-silicotic effect.