Design, synthesis and biological activity evaluation of novel antibacterial agent (E)-1-(4-chlorobenzyl)-5-styrylindoline-2, 3-dione

Isatin, an endogenous compound existent in many organisms, shows a wide range of biological activities. In this paper, we like to report the design and synthesis of several isatin derivatives through the acetalization, N-Alkylation, Microwave-asistant Heck coupling reaction and so on. Among those compounds, (E)-1-(4-chlorobenzyl)-5-styryl-indoline-2-carbonyl-3, 3-(ethylenedioxy) dione and (E)-1-(4-chlorob-enzyl)-5-styrylindoline-2, 3-dione possess novel structures and have not been reported before. Five newly synthesized compounds were characterized on the basis of ¹H NMR, ¹³C NMR and mass spectra, and those compounds were tested for their biological activities against Gram⁺ and Gram^- bacteria. Among those compounds, (E)-1-(4-chlorobenzyl)-5-styrylindoline-2, 3-dione demonstrated good antibacterial activity against Escherichia coli (Gram^-) and moderate antibacterial activity against Staphyloccocus aureus (Gram⁺).