ER-a36 participate growth inhibiting which induced by Tamoxifen in gastric cancer cell line BGC823

Author

Deng Hao ; Wang Xuming ; Xuan Huang ; Hongyan Zhen ; Ying Chen ; Lijiang Liu ; Zhaoyi Wang

Author_Institution

Dept. of Pathology & Pathophysiology, Jianghan Univ., Wuhan, China

fYear

2011

fDate

19-22 Aug. 2011

Firstpage

1062

Lastpage

1065

Abstract

We aimed to investigate the expression and location change of ER-a36 in BGC823 when dealt with TAM. Cell growth was examined by WST-1 and assayed by microplate reader. ER-a36 expressions in mRNA and protein were assayed by RT-PCR and Western-blot method and were quantitated. The location of ER-α36 in cell was examined by immunofluorenscence methods. The cell numbers significant decreased at all TAM treatment groups compared with control group and cell growth rate had a concentration-dependent of TAM. ER-α36 mRNA and protein expressions decreased at all TAM treatment groups compared with control group and had a concentration-dependent of TAM. ER-α36 is localized on the membrane and cytoplasm of BGC823 cell, and its location did not change when dealt with TAM TAM could inhibit the growth of BGC823 cell, and ER-α36 may transmit growth regulated signal of TAM through a membrane-based pathway in gastric cancer.

Keywords

biomembranes; cancer; cellular biophysics; fluorescence; molecular biophysics; patient treatment; proteins; ER-a36 participate growth; Tamoxifen; WST-1; Western-blot method; cell growth; cytoplasm; gastric cancer cell line BGC823; immunofluorenscence methods; mRNA; membrane; microplate reader; protein; Biomembranes; Breast cancer; Educational institutions; Humans; Proteins; RNA; BGCS23 cell line; ER-α36; Gastric cancer; Growth; Tamoxifen;

fLanguage

English

Publisher

ieee

Conference_Titel

Human Health and Biomedical Engineering (HHBE), 2011 International Conference on

Conference_Location

Jilin

Print_ISBN

978-1-61284-723-8

Type

conf

DOI

10.1109/HHBE.2011.6029007

Filename

6029007