Comparison of effects on the anti-metastasis between isoliquiritigenin and glabridin

The aim of this study was to evaluate the anti-metastasis effects and the inhibition of the tube-like structure formation of human umbilical vein endothelial cells ECV304 of isoliquiritigenin and glabridin. The cell survival ratio of mice melanoma cells B16F1 was measured by SRB assay, the lethality rate was tested by trypan blue exclusion test. The survival ratio of human umbilical vein endothelial cells ECV304 were measured by SRB and AO/EB assays. The migration of B16F1 cells and ECV304 cells were measured by scratch wound assay. The matrix metalloproteinase-2 (MMP-2) activity in B16F1 cells culture medium was measured by gelatin zymography and ELISA methods. The inhibition on tube-like structure formation of human umbilical vein endothelial cells ECV304 were detected on artificial basilar membrane rebuilt by matrigel. Taken together, our data suggested that isoliquiritigenin and glabridin have strong anti-metastasis activities, in the same concentration, isoliquiritigenin has stronger function.