The changes of acid-sensing ion channel 1a in focal cerebral ischemic diabetes rats and neuroprotection of fasudil

The symptoms of diabetes focal ischemia were more serious, but the concrete machanism is not very clear. For this we measure the expression of Acid - sensing ion channel 1a (ASIC1a) in ischemic cortex of diabetic rats during different time, meanwhile observe the protection of fasudil after ischemia. 72 male wistar rats were divided into 3 groups: focal ischemia, diabetes focal ischemia and fasudil interfere, ischemia 1h, 3h, 6h, 24h per group. high fat diet plus streptozotocin to model diabetic, thread cork to establish the models of focal ischemia. interfere group received 1mg/Kg fasudil injection by tail vein during half an hour after making diabetic model. The expressions of ASIC1a were detected by Western Blot. the results indicated diabetes groups were serious, ASIC1a were increasing and time-dependent (P<;0.05); After fasudil, ASIC1a decreased significantly. So the aggravated cause of diabetes ischemia maybe associate with excessive opening of ASIC1a., Fasudil is neuroprotective by inhibit the expression of ASIC1a.