Title :
Multi-modality non-linear microscopy
Author :
Chi-Kuang Sun ; Shi-Wei Chu ; I-Shiu Chen ; Tze-Min Liu ; Ping-Chin Cheng ; Bai-Ling Lin
Author_Institution :
Graduate Inst. of Electro-Optical Eng., Nat. Taiwan Univ., Taipei, Taiwan
Abstract :
Summary form only given. Two-photon fluorescence microscopy (TPFM) meets wide applications in biology with high axial resolution without using a confocal aperture. In TPFM, strong fluorescence emission is restricted to the vicinity of the focal point with the advantage of minimum out-of-focus absorption, photo-bleaching and damage. The use of near infrared (NIR, generally around 800 nm) leads to deeper penetration in most biological specimens than conventional light sources, providing an opportunity for tissue imaging.
Keywords :
biological techniques; biological tissues; cellular biophysics; fluorescence; molecular biophysics; optical microscopy; optical saturable absorption; two-photon processes; 800 nm; biological specimens; biological structures; biology; confocal aperture; conventional light sources; damage; fluorescence emission; fluorescent stains; multi-modality nonlinear microscopy; photo-bleaching; tissue imaging; two-photon fluorescence microscopy; Absorption; Biology; Fluorescence; Laser excitation; Laser transitions; Light sources; Optical attenuators; Solid lasers; Sun; Transmission electron microscopy;
Conference_Titel :
Lasers and Electro-Optics, 2001. CLEO '01. Technical Digest. Summaries of papers presented at the Conference on
Conference_Location :
Baltimore, MD, USA
Print_ISBN :
1-55752-662-1
DOI :
10.1109/CLEO.2001.947731
