Imaging intracellular Ca/sup 2+/ dynamic with third harmonic generation microscope

Author

Canioni, L. ; Rivet, S. ; Sarger, Laurent ; Barille, R. ; Vacher, P. ; Voisin, P.

Author_Institution

Centre de Phys. Moleculaire et ondes Hertziennes, Talence, France

fYear

2001

fDate

11-11 May 2001

Firstpage

222

Lastpage

223

Abstract

Summary form only given. The validity of the third harmonic generation (THG) microscopy for imaging has been shown and its potential use in material science and more precisely in biology. All these studies have demonstrated that THG microscopy is a method which has the advantage that no labeling is necessary. In order to develop further imaging instruments, biological dynamic events has to be measured and especially Ca/sup 2+/ dynamics which is a special importance in cellular biology. For the biological experiments, we have used a human astrocytome cell line: U-87 MG.

Keywords

biological techniques; biological tissues; calcium; cellular transport; optical harmonic generation; optical microscopes; optical microscopy; Ca/sup 2+/; Ca/sup 2+/ dynamics; THG microscopy; U-87 MG; biological dynamic events; biological experiments; biology; cellular biology; human astrocytome cell line; imaging; imaging instruments; intracellular Ca/sup 2+/; material science; third harmonic generation microscope; Absorption; Fluorescence; Frequency conversion; Laser excitation; Laser transitions; Light sources; Optical attenuators; Solid lasers; Sun; Transmission electron microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2001. CLEO '01. Technical Digest. Summaries of papers presented at the Conference on

Conference_Location

Baltimore, MD, USA

Print_ISBN

1-55752-662-1

Type

conf

DOI

10.1109/CLEO.2001.947732

Filename

947732