Separation of tumor cells from bronchoalveolar lavage fluid (BALF) via microgrooves enhanced immiscible filtration assisted by surface tension (ME-IFAST)

Author

Yongbing Chen ; Jun Zhao ; Yaoping Liu ; Menghe Yuan ; Wei Wang ; Ming Chu ; Yuedan Wang ; Haichao Li

Author_Institution

Inst. of Microelectron., Peking Univ., Beijing, China

fYear

2013

fDate

16-20 June 2013

Firstpage

309

Lastpage

312

Abstract

Separation and characterization of lung cancer cells is of great importance to the diagnosis and treatment assessment of this disease. Here, we reported a microgrooves-enhanced IFAST strategy (ME-IFAST) for tumor cell separation from clinical BALF sample. After the process, we enhanced the efficiency of beads bonded on each cell, from around 14.6 to 28.8 beads per cell, and decreased the number of un-bonded beads by 30.2% as well as cells with no bonded beads. It has been proved to be an efficient size amplification performance with a low free magnetic beads background.

Keywords

bioMEMS; biomagnetism; cancer; cellular transport; filtration; lung; magnetic particles; patient diagnosis; patient treatment; pneumodynamics; separation; surface tension; tumours; ME-IFAST; bronchoalveolar lavage fluid; clinical BALF sample; disease diagnosis; low free magnetic bead background; lung cancer cell characterization; lung cancer cell separation; microgrooves enhanced immiscible filtration assisted by surface tension; microgrooves-enhanced IFAST strategy; size amplification performance; treatment assessment; tumor cell separation; Cancer; Fluids; Loading; Lungs; Magnetic separation; Suspensions; Tumors; BALF; ME-IFAST; cell sorting; lung cancer;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on

Conference_Location

Barcelona

Type

conf

DOI

10.1109/Transducers.2013.6626764

Filename

6626764