Integrated analysis of hela cell microRNAs´ microarray data and anti-cancer pathway prediction of beta-sitosterol

It has remarkable efficacy to treat cervical cancer cell using beta-sitosterol. At present, beta-sitosterol has been used in the clinical treatment of cancer and its side effects are remotely lower than the traditional drug such as cisplatin. However, the anticancer mechanism of beta-sitosterol is unknown in cervical cancer. In this study, we mainly use TaqMan MicroRNA Assay to detect the consequence of Hela cell treatment by beta-sitosterol and cisplatin and analyze it from molecular level. We find out the differentially expressed microRNAs and forecast their targets by Targetscan. We also provide direct evidence that STK4/MST1 is a functional target. From the viewpoint of bioinformatics, we speculate the possible pathway of beta-sitosterol to inhibit tumor.