DocumentCode :
3454208
Title :
A novel oxygen tension programmable microfluidic system (oPROMs) for in vitro cell biology studies
Author :
Rajan, Niju ; Narayan, Ananth ; Wu, Zhisheng ; Wu, Po-Han ; Ahn, Chong H. ; Narayan, R.K. ; Li, Cong
Author_Institution :
Cushing Neuromonitoring Lab., Feinstein Inst. for Med. Res., New York, NY, USA
fYear :
2013
fDate :
16-20 June 2013
Firstpage :
412
Lastpage :
415
Abstract :
Creating different oxygen tensions at a microscale level can be beneficial for studies that investigate oxygen-dependent cellular behavior. Here we present a novel, highly flexible, oxygen tension programmable microfluidic system (oPROMs) capable of generating various uniform oxygen tensions without using complex device structures and multiple gas interconnections. The oPROMs device is coated with a mixture of Glucose oxidase (GOX) and Catalase (CAT) immobilized in a chitosan matrix and then covered by membranes with different thickness. The high glucose medium used in cell culture interacts with the enzyme layer and produces a steady hypoxia inside the wells. Based on different combination of GOX and CAT, oxygen tensions from 0% to 7% were produced.
Keywords :
biological techniques; enzymes; microfluidics; sugar; GOX; Glucose oxidase; cell culture; chitosan matrix; enzyme layer; immobilized Catalase; in vitro cell biology studies; oPROMs; oxygen tension programmable microfluidic system; uniform oxygen tension; Biochemistry; Hydrogen; Media; Microchannel; Microfluidics; Oxygen; Sugar; Oxygen tension; cell culture device; hypoxia; microfluidic; oxygen gradient;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on
Conference_Location :
Barcelona
Type :
conf
DOI :
10.1109/Transducers.2013.6626790
Filename :
6626790
Link To Document :
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