Circadian rhythms in neurospora crassa on a microfluidic device for real-time gas perturbations

Racetubes, conventional system employing hollow glass tubes, are typically used for monitoring circadian rhythms from a model filamentous fungus Neurospora crassa. However, a major technical limitation in using the conventional system is that this racetube is not amenable for real-time gas perturbations. In this work, a simple yet efficient microfluidic device with real-time gas perturbations has been proposed and developed for monitoring the variations of circadian rhythms for Neurospora crassa using bioluminescence assays. The developed platform is a useful toolbox for the investigation of molecular responses under various gas conditions for Neurospora and also can be applied to other microorganisms.