FRET imaging by picosecond TCSPC laser scanning microscopy

Author

Becker, W. ; Bergmann, A. ; Benndorf, K. ; Biskup, C. ; Zimmer, T.

Author_Institution

Becker & Hickl GmbH, Berlin, Germany

fYear

2001

fDate

11-11 May 2001

Abstract

Summary form only given. Two-photon laser scanning microscopy and a new TCSPC imaging technique was used to obtain FRET intensity-lifetime images of living cells. Double exponential decay analysis separated the FRET fluorescence from the fluorescence of the unquenched acceptor molecules.

Keywords

biological techniques; cellular biophysics; fluorescence; high-speed optical techniques; optical microscopy; photon counting; radiative lifetimes; two-photon processes; FRET fluorescence; confocal laser scanning microscope; double exponential decay analysis; fluorescence intensity; fluorescence lifetime; high count rate; intensity-lifetime images; large histogram memory; living cells; low differential nonlinearity; picosecond laser scanning microscopy; reversed start-stop configuration; scanning interface; simultaneous two-photon excitation; time-correlated single photon counting imaging; time-resolved FRET images; two-photon microscopy; Clocks; Detectors; Fluorescence; Histograms; Laser excitation; Microscopy; Optical pulses; Photomultipliers; Physiology; Titanium;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2001. CLEO '01. Technical Digest. Summaries of papers presented at the Conference on

Conference_Location

Baltimore, MD, USA

Print_ISBN

1-55752-662-1

Type

conf

DOI

10.1109/CLEO.2001.948243

Filename

948243