Title :
Molecular Cloning and Expression of Ppdc-ldhL Fusion Gene in Zymomonas Mobilis ATCC 10988
Author :
Gao, Nian-Fa ; Wang, Yong ; Gao, Qiang ; Wang, Bin-Zhe ; Zhang, Jian ; Liu, Bo-Ya
Author_Institution :
Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin, China
Abstract :
In this study, the 5´ terminus of ldhL gene in Lactobacillus casei ATCC 334 was fused to the 3´ terminus of pdc gene promoter (Ppdc) part in Zymomonas mobilis ATCC 10988 by recombinant PCR. The fusion gene Ppdc-ldhL was integrated in broad-host-range plasmid pBBR1MCS3, transformed by electroporation and expressed in Z. mobilis ATCC 10988. The primary expression of heterologous lactate dehydrogenase in Z. mobilis lays the foundation to expand the application range of higher value products by Z. mobilis.
Keywords :
biochemistry; enzymes; genetics; microorganisms; molecular biophysics; oxidation; solvation; Lactobacillus casei ATCC 334; Ppdc-ldhL fusion gene; Ppdc-ldhL integration; Z. mobilis; Zymomonas mobilis ATCC 10988; broad-host-range plasmid pBBR1MCS3; electroporation; lactate dehydrogenase; molecular cloning; molecular expression; Biochemistry; Bioinformatics; Biotechnology; Capacitive sensors; Chemical industry; Cloning; DNA; Ethanol; Nitrogen; Production; L-lactate dehydrogenase; Lactobacillus casei; Zymomonas mobilis; electroporation; pBBR1MCS3;
Conference_Titel :
Bioinformatics, Systems Biology and Intelligent Computing, 2009. IJCBS '09. International Joint Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-0-7695-3739-9
DOI :
10.1109/IJCBS.2009.108
