Radical induced protein crystallization by radical amplification microfluidic chip

The paper reports plasma induced radical concentration microfluidic chip and radical induced protein crystallization. The novelty of our work is collection and concentration of radical species and which is confirmed by ESR (Electron Spin Resonance) measurement. Formation of bubbles which has a relatively large surface area accelerates the production of radical species at air-liquid interface and these bubbles are concentrated by the circulating flow in the microfluidic chip. It was also confirmed that the radical-induced protein crystal are produced with increasing of the circulation time. This microfluidic chip contributes to the protein crystal production and bio-medical applications.