Title :
Multicolor ultra high throughput parallel microfluidic flow cytometer
Author :
Fan, Y.J. ; Chen, Yuanfeng ; Wu, Y.C. ; Kung, Y.C. ; Wu, T.H. ; Sheen, H.J. ; Chiou, P.Y.
Author_Institution :
Univ. of California Los Angeles, Los Angeles, CA, USA
Abstract :
We report an ultrahigh throughput multi-color microfluidic flow cytometer with 32 detection channels and 64 sheath-flow channels. This flow cytometer is realized by integrating a high N.A. microball lens array with a 3D microfluidic device, which allows introducing cell samples into 32 sample channels, and sheath flows into 64 channel for parallel microfluidic focusing using only two inlets. A throughput of 384,000 cell/sec has been accomplished on a single device.
Keywords :
bioMEMS; biomedical equipment; cellular biophysics; microfluidics; microlenses; 3D microfluidic device; cell samples; detection channels; high N.A. microball lens array; multicolor ultrahigh throughput parallel microfluidic flow cytometer; parallel microfluidic focusing; sheath-flow channels; Arrays; Fluorescence; Focusing; Lenses; Microfluidics; Optical sensors; Throughput; BioMEMS; FACS; flow cytometry; microfluidics;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on
Conference_Location :
Barcelona
DOI :
10.1109/Transducers.2013.6627327
