Long-term, high-spatiotemporal resolution recording from cultured organotypic slices with high-density microelectrode arrays

Author

Gong, W. ; Sencar, J. ; Jackel, D. ; Muller, J. ; Fiscella, M. ; Radivojevic, M. ; Bakkum, D. ; Hierlemann, A.

Author_Institution

Dept. of Biosyst. Sci. & Eng., ETH Zurich, Zurich, Switzerland

fYear

2015

fDate

21-25 June 2015

Firstpage

1037

Lastpage

1040

Abstract

A novel system to cultivate and record brain slices directly on high-density microelectrode arrays (HD-MEA) was developed. This system allows to continuously record electrical activity of selected individual neurons at high spatial resolution, while monitoring neuronal network activity at the same time. For the first time, properties of single neurons and the corresponding neuronal network in an organotypic hippocampal slice culture were studied over four consecutive weeks at daily intervals.

Keywords

bioelectric phenomena; brain; microelectrodes; neurophysiology; brain slice cultivation; brain slice recording; cultured organotypic slices; electrical activity; high density microelectrode arrays; high spatiotemporal resolution recording; neuronal network activity; neurons; Biological neural networks; Extracellular; Microelectrodes; Neurons; Spatial resolution; Surface impedance; High-density microelectrode arrays; long-term experiment; organotypic hippocampal slice; single neuron tracing;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2015 Transducers - 2015 18th International Conference on

Conference_Location

Anchorage, AK

Type

conf

DOI

10.1109/TRANSDUCERS.2015.7181103

Filename

7181103