Dynamic MCP-1 mRNA response of human aortic endothelial cells (HAEC) to step changes in laminar shear stress

Author

Guild, J.B. ; Chromiak, A.B. ; Sprague, E.A. ; Nerem, R M

Author_Institution

Inst. of Bioeng. & Biosci., Georgia Inst. of Technol., Atlanta, GA, USA

Volume

1

fYear

1999

fDate

1999

Abstract

Our aim is to study the hemodynamic alteration of monocyte recruitment and adhesion in model blood vessel systems, focusing on the hemodynamic effects of the recruitment factor monocyte chemotactic protein one, MCP-1, gene expression in HAEC. Preconditioning with low shear stress, LS, elevates MCP-1 gene activity and eliminates any transient peak in activity upon switching to high shear stress, HS, without affecting HS-induced gene down-regulation. These data suggest an interaction between the pathways mediating the long term response to LS and the transient cellular response to HS onset

Keywords

adhesion; blood vessels; cellular biophysics; haemodynamics; haemorheology; adhesion; arterial tissue; dynamic MCP-1 mRNA response; gene activity; gene down-regulation; gene expression; hemodynamic alteration; human aortic endothelial cells; laminar shear stress; long term response; model blood vessel systems; monocyte chemotactic protein one; monocyte recruitment; step changes; transient cellular response; Biomedical engineering; Chemical technology; Gene expression; Hemodynamics; Humans; In vivo; RNA; Recruitment; Stress; Switches;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.802021

Filename

802021