Directional secretion of human growth hormone by genetically-modified endothelial cells

Author

Gusic, R. ; Sieminski, A.L. ; Gooch, K.J.

Author_Institution

Dept. of Bioeng., Pennsylvania Univ., Philadelphia, PA, USA

Volume

1

fYear

1999

fDate

1999

Abstract

Endothelial cells, which line the lumen of blood vessels, are in a useful location to deliver proteins to the blood. The authors have studied the directional secretion of human growth hormone (hGH) by genetically-modified endothelial cells and have developed a mathematical model which can further be applied to describe the delivery of larger proteins to the circulation. By fitting experimental data to the mathematical model of their system, the authors estimate apical and basal hGH production rates of 4.3±0.4 and 7.4±0.5 ng/h per 1E6 cells, respectively (mean±stdev, N=4)

Keywords

biochemistry; blood vessels; cellular transport; genetics; physiological models; proteins; blood vessels lumen; directional secretion; genetically-modified endothelial cells; human growth hormone; mathematical model; proteins delivery to blood; Biochemistry; Biomembranes; Blood; Fluids and secretions; Humans; Implants; Mathematical model; Mice; Production; Proteins;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.802034

Filename

802034