Radiation effect on the permeability and cell adhesion of the cerebral microvasculature

Author

Yuan, H. ; Gaber, M.W. ; Naimark, M. ; Merchant, T.E. ; Kiani, M.F.

Author_Institution

Sch. of Biomed. Eng., Tennessee Univ., Memphis, TN, USA

Volume

1

fYear

1999

fDate

1999

Abstract

The aim was to evaluate the effect of irradiation on cerebral microvascular permeability and cell adhesion. A cranial window was implanted in anesthetized male Sprague-Dawley rats to visualize the cerebral subpial vessels. Intravital fluorescence microscopy was used to obtain the fluorescent images of cerebral microvessels after i.v injection of FITC-dextran and rhodamine to measure vascular permeability and leukocyte adhesion, respectively. Permeability was determined by measurement of relative fluorescent intensity inside and outside of venules at specific time points after irradiation

Keywords

biological effects of ionising radiation; biological techniques; blood vessels; brain; cellular effects of radiation; fluorescence; optical microscopy; FITC-dextran; anesthetized male Sprague-Dawley rats; cell adhesion; cerebral microvascular permeability; cerebral microvessels; cerebral subpial vessels; cranial window; fluorescent images; intravital fluorescence microscopy; irradiation; leukocyte adhesion; radiation effect; relative fluorescent intensity; rhodamine; vascular permeability; venules; Adhesives; Cranial; Fluorescence; Microscopy; Permeability measurement; Radiation effects; Rats; Time measurement; Visualization; White blood cells;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.802043

Filename

802043