Visualization of neutrophil-platelet tether dynamics under flow

Author

Schmidtke, David W. ; Diamond, Scott L.

Author_Institution

Dept. of Chem. Eng., Pennsylvania Univ., Philadelphia, PA, USA

Volume

1

fYear

1999

fDate

1999

Abstract

Adhesion and aggregation of platelets and neutrophils to each other and to the vessel wall are critical events in inflammation and thrombosis. Using a novel microslide flow chamber, we have performed high speed (240 frames per second), high resolution (63X, 1.4 NA) differential interference contrast (DIC) videomicroscopy of the short-lived adhesive interactions between individual neutrophils and platelets under flow. We detected short-lived (150-700 msec), membrane tethers (1-20 μm) between free-flowing neutrophils (wall shear rate=100 to 300 s^-1) and spread platelet, consistent with the half-life of the P-selectin/PSGL-1 bond

Keywords

adhesion; aggregation; biomembranes; blood; cellular biophysics; haemorheology; optical microscopy; 1 to 20 micron; 150 to 700 ms; P-selectin/PSGL-1 bond; adhesion; aggregation; differential interference contrast videomicroscopy; free-flowing neutrophils; half-life; high resolution; high speed; inflammation; membrane tethers; microslide flow chamber; neutrophil-platelet tether dynamics; short-lived adhesive interactions; spread platelet; thrombosis; vessel wall; visualization under flow; Adhesives; Biomembranes; Bonding; Chemical engineering; Engine cylinders; Humans; Interference; Microscopy; Petroleum; Visualization;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.802078

Filename

802078