Electroporation of single cells by high frequency shocks

Author

Bilska, Anna O. ; DeBruin, Katherine A. ; Krassowska, Wanda

Author_Institution

Dept. of Biomed. Eng., Duke Univ., Durham, NC, USA

Volume

1

fYear

1999

fDate

1999

Abstract

This modeling study investigates electroporation in a single cell exposed to a 10 ms pulse train consisting of high frequency monophasic or biphasic shocks. The fraction of the cell membrane occupied by pores decreases with frequency, and the decrease is more gradual for monophasic pulses. This result suggests that varying the frequency of the monophasic pulse train may provide control over the uptake of molecules during electroporation-mediated drug and gene therapies

Keywords

bioelectric phenomena; biomembrane transport; drug delivery systems; porosity; shock wave effects; 10 ms; 200 kHz to 6 MHz; Laplace equation; biphasic shocks; cell membrane; drug therapy; electroporation; frequency variation; gene therapy; high frequency shocks; ionic channels; monophasic shocks; pore density; pulse train exposed cell; single cell; transmembrane current; uptake of molecules control; Biomembranes; Cells (biology); Differential equations; Drugs; Electric shock; Extracellular; Frequency; Gene therapy; Laplace equations; Microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint

Conference_Location

Atlanta, GA

ISSN

1094-687X

Print_ISBN

0-7803-5674-8

Type

conf

DOI

10.1109/IEMBS.1999.802122

Filename

802122