Title :
Direct measurement of force between a bacterium and a flat surface
Author :
Clapp, Aaron R. ; Truesdail, S.E. ; Dickinson, Richard B.
Author_Institution :
Dept. of Chem. Eng., Florida Univ., Gainesville, FL, USA
Abstract :
The authors have developed a novel technique to directly measure the long-range interaction forces between a single bacterium and a surface. The technique implements a single-beam gradient optical trap as a simultaneous micropositioner and force transducer, and evanescent wave light scattering as a means of measuring the cell-surface separation distance. The technique has measured the interaction forces between a single S. aureus bacterium and a surface as a function of separation distance
Keywords :
bio-optics; biological techniques; biomechanics; cellular biophysics; distance measurement; force measurement; light scattering; microorganisms; radiation pressure; bacterium-flat surface force measurement; biophysical technique; cell-surface separation distance measurement; evanescent wave light scattering; force transducer; long-range interaction forces; micropositioner; separation distance; single S. aureus bacterium; single-beam gradient optical trap; Atomic force microscopy; Biomedical measurements; Biomedical optical imaging; Charge carrier processes; Force measurement; Light scattering; Microorganisms; Optical scattering; Optical sensors; Optical surface waves;
Conference_Titel :
[Engineering in Medicine and Biology, 1999. 21st Annual Conference and the 1999 Annual Fall Meetring of the Biomedical Engineering Society] BMES/EMBS Conference, 1999. Proceedings of the First Joint
Conference_Location :
Atlanta, GA
Print_ISBN :
0-7803-5674-8
DOI :
10.1109/IEMBS.1999.803896
