Title :
Fluorescence microscopy without resolution limit
Author :
Gustafsson, Mats G L ; Shao, Ling ; Agard, D.A. ; Sedat, J.W.
Author_Institution :
Dept. of Physiol., California Univ., San Francisco, CA, USA
Abstract :
The diffraction limit to light microscope resolution, which was seen as an impassable barrier for a century, has recently been broken. Structured illumination light in combination with nonlinear phenomena allow theoretically unlimited resolution.
Keywords :
fluorescence; image resolution; light diffraction; lighting; optical microscopy; diffraction limit; fluorescence microscopy; light microscope resolution; nonlinear phenomena; resolution limit; structured illumination light; Biochemistry; Biomedical engineering; Diffraction; Fluorescence; Frequency; Labeling; Lighting; Optical microscopy; Physiology; Spatial resolution;
Conference_Titel :
Biophotonics/Optical Interconnects and VLSI Photonics/WBM Microcavities, 2004 Digest of the LEOS Summer Topical Meetings
Print_ISBN :
0-7803-8306-0
DOI :
10.1109/LEOSST.2004.1338662
