Title :
Optical biosensors from chemical transduction by lectins and neural receptors
Author :
Krull, Ulrich J. ; Brown, R. Stephen ; Vandenberg, Elaine T. ; Hougham, B.D.
Author_Institution :
Dept. of Chem., Toronto Univ., Mississauga, Ont., Canada
Abstract :
Instrumentation for optrode development in an intrinsic configuration was demonstrated by fluorescence from pyrene-labeled concanavalin A absorbed to the surface of an optical fiber. A system showing sensitivity, high selectivity, and reversibility was derived from the fluorescence detection of acetylcholine receptor binding to agonist. Fluorescence enhancement was seen for systems where a fluorophore was nonspecifically labeled to the receptor/membrane structure and also where only the lipid was fluorescent.<>
Keywords :
biological techniques and instruments; fibre optic sensors; agonist; chemical transduction; fluorescence; fluorophore; lectins; neural receptors; optical biosensors; optical fiber; optrode development instrumentation; pyrene-labeled concanavalin A;
Conference_Titel :
Engineering in Medicine and Biology Society, 1988. Proceedings of the Annual International Conference of the IEEE
Conference_Location :
New Orleans, LA, USA
Print_ISBN :
0-7803-0785-2
DOI :
10.1109/IEMBS.1988.95045
