Title :
Cell membrane fibers for the platform of transmembrane protein analysis
Author :
Sato, Kiminori ; Takeuchi, Shoji
Author_Institution :
Inst. of Ind. Sci., Univ. of Tokyo, Tokyo, Japan
Abstract :
Transmembrane proteins functioning as the cellular signaling gateway are the major molecular targets of commercial drug therapy. HEK293T cells are world widely used cell based-platform for these proteins analysis. We loaded the photo-curable hydrogel into the HEK293T cells, treated with UV through the micron photo-mask, and fabricated the cell membrane fiber. The activity of ion channels on the surface of fiber was recorded by using patch-clamp technique. The structure of cell membrane fiber is the similar with that of axial fibers. Structural stability of the core polymer in CMFs will allow the complex analysis of ion channel activity.
Keywords :
bioelectric phenomena; biomembrane transport; cellular biophysics; curing; drug delivery systems; drugs; hydrogels; masks; molecular biophysics; molecular configurations; photochemistry; polymer structure; proteins; HEK293T cells; axial fibers; cell based-platform; cell membrane fibers; cellular signaling gateway; commercial drug therapy; core polymer; ion channel activity; micron photomask; molecular targets; patch-clamp technique; photocurable hydrogel; structural stability; transmembrane protein analysis; transmembrane proteins functioning; Biomembranes; Drugs; Electrodes; Fluorescence; Glass; Polymers; Proteins;
Conference_Titel :
Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on
Conference_Location :
Taipei
Print_ISBN :
978-1-4673-5654-1
DOI :
10.1109/MEMSYS.2013.6474387
