Fabrication of micro pore arrays in free standing COC membranes and their application for in vitro barrier tissue models

Author

Gel, M. ; Kandasamy, Sivasathivel ; Cartledge, K. ; Haylock, D.

Author_Institution

Mater. Sci. & Eng., CSIRO, Clayton, VIC, Australia

fYear

2013

fDate

20-24 Jan. 2013

Firstpage

895

Lastpage

898

Abstract

This paper reports a low-cost fabrication method for mass production of free standing cyclic olefin copolymer (COC) membranes embedded with micro pore arrays. We show that the method creates suspended membranes with thickness of 2μm and micro pores having diameter ranging from 3-5μm. We integrated the membrane in a microfluidic system designed to mimic the endothelial barrier in bone marrow sinusoids. The device was tested with HUVEC and supported their attachment and proliferation on the membrane.

Keywords

bioMEMS; biological tissues; biomedical materials; biomembranes; bone; cellular biophysics; microfluidics; molecular biophysics; polymer blends; porosity; tissue engineering; HUVEC; bone marrow sinusoids; endothelial barrier; free standing cyclic olefin copolymer membranes; in vitro barrier tissue models; low-cost fabrication method; mass production; membrane attachment; membrane proliferation; microfluidic system; micropore array fabrication; size 2 mum; size 3 mum to 5 mum; suspended membranes; Biomembranes; Bones; Cells (biology); Fabrication; Films; In vitro; Microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on

Conference_Location

Taipei

ISSN

1084-6999

Print_ISBN

978-1-4673-5654-1

Type

conf

DOI

10.1109/MEMSYS.2013.6474388

Filename

6474388