Impedance biosensor based on interdigitated electrode arrays for detection of low levels of E.coli O157:H7

Author

Dastider, S.G. ; Barizuddin, S. ; Wu, Yaowu ; Dweik, M. ; Almasri, M.

Author_Institution

Dept. of Electr. & Comput. Eng., Univ. of Missouri, Columbia, MO, USA

fYear

2013

fDate

20-24 Jan. 2013

Firstpage

955

Lastpage

958

Abstract

We report an interdigitated electrode array (IDEA) impedance biosensor with rapid detection of Escherichia coli O157:H7. This biosensor has significantly better detection capability compared with the conventional microbiological testing method. The total detection time for this biosensor is under 3 hours. Dielectrophoresis (DEP) is used in conjunction with impedance spectroscopy to improve the detection capability of the biosensor. The IDEA surface is functionalized using anti-E.coli antibody for selectivity of the bacteria. The dose response was in-between 3 × 10⁶ CFU/mL and 3 × 10² CFU/mL. This is 10 fold better than the detection limit previously reported using impedance based biosensors.

Keywords

biosensors; electrochemical electrodes; electrochemical impedance spectroscopy; electrophoresis; microorganisms; Escherichia coli O157:H7 detection; antiE.coli antibody; conventional microbiological testing method; detection capability; dielectrophoresis; dose response; impedance biosensor; impedance spectroscopy; interdigitated electrode arrays; time 3 h; total detection time; Biosensors; Electrodes; Focusing; Impedance; Microchannel; Microorganisms; Surface impedance;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2013 IEEE 26th International Conference on

Conference_Location

Taipei

ISSN

1084-6999

Print_ISBN

978-1-4673-5654-1

Type

conf

DOI

10.1109/MEMSYS.2013.6474404

Filename

6474404