Temporal observation of apoptosis in tumor tissues via electrochemical treatment (EchT)

Summary form only given. Growth, arrest and suicide (apoptosis) of cells are all legitimate responses associated with DNA damage, although the choice of fate in each instance depends upon the cell type, location, environment, and extent of its damage. Apoptosis is a fundamental mode of cell death, which performs a regulatory function during normal development, in tissue homeostasis, and in some disease processes. Apoptosis may be the prudent option in damaged cells that retain substantial replicative potential and therefore constitute a neoplastic risk. To induce apoptosis, various means are chosen among the X-ray, UV-radiation, anti-tumor drugs, and direct current, the last of which is the choice of tool for this paper for study of tumor tissues such as tumor regression and its necrosis. The modality of direct current in apoptosis has gained vast interest lately, Temporal variation of apoptosis by means of direct current will provide a clue to the series of immune reaction and thus deserve a careful study, in addition to its usefulness to determine the quantitative amount of temporal DC current in the clinical trials. A stimulating system consists of power supply, Pt-electrodes, 6-well plates, etc. To ablate adsorption and desorption of electrode materials to directly affect tumor cells or its tissues and perform faradaic processes on electrodes, electrodes of pure platinum are used.