Spatially-variant Lucy-Richardson deconvolution for multiview fusion of microscopical 3D images

Author

Temerinac-Ott, Maja ; Ronneberger, Olaf ; Nitschke, Roland ; Driever, Wolfgang ; Burkhardt, Hans

Author_Institution

Inst. of Comput. Sci., Albert-Ludwigs-Univ. of Freiburg, Freiburg, Germany

fYear

2011

fDate

March 30 2011-April 2 2011

Firstpage

899

Lastpage

904

Abstract

A framework for fast multiview fusion of Single Plane Illumination Microscopy (SPIM) images based on a spatially-variant point spread function (PSF) model is presented. For the multiview fusion a new algorithm based on the regularized Lucy-Richardson deconvolution and the Overlap-Save method is developed and tested on SPIM images. In the algorithm the image is decomposed into small blocks which are processed separately thus saving memory space and allowing for parallel processing.

Keywords

biomedical optical imaging; deconvolution; image fusion; medical image processing; optical microscopy; optical transfer function; SPIM; microscopical 3D images; multiview fusion; overlap-save method; parallel processing; point spread function; single plane illumination microscopy; spatially-variant Lucy-Richardson deconvolution; Deconvolution; Image reconstruction; Image restoration; Microscopy; Noise; Shape; TV; Lucy-Richardson deconvolution; SPIM; spatially-variant PSF; total variation;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: From Nano to Macro, 2011 IEEE International Symposium on

Conference_Location

Chicago, IL

ISSN

1945-7928

Print_ISBN

978-1-4244-4127-3

Electronic_ISBN

1945-7928

Type

conf

DOI

10.1109/ISBI.2011.5872549

Filename

5872549