Title :
Stochastic 3-D signal reconstruction from noisy projection data for heterogeneous instances of objects in electron microscopy imagery
Author :
Zheng, Yili ; Wang, Qiu ; Doerschuk, Peter C.
Author_Institution :
Comput. Res. Div., Lawrence Berkeley Nat. Lab., Berkeley, CA, USA
fDate :
March 30 2011-April 2 2011
Abstract :
Different instances of a biological macromolecular complex need not share the same 3-D electron scattering distribution function due to stoichiometric variability, flexibility and vibrations, etc. Cryo electron microscopy provides 2-D images of each of many such complexes where each image is roughly a projection. A statistical estimation problem is described and demonstrated for determining a statistical description of the complex from such imagery.
Keywords :
biological techniques; biology computing; biothermics; electron microscopy; macromolecules; molecular biophysics; molecular configurations; signal processing; stochastic processes; 3D electron scattering distribution function; biological macromolecular complex; cryoelectron microscopy; electron microscopy imagery; heterogeneous object instances; noisy projection data; statistical estimation problem; stochastic 3D signal reconstruction; Biology; Covariance matrix; Electron microscopy; Image reconstruction; Maximum likelihood estimation; USA Councils; 3-D image reconstruction; cryo electron microscopy; maximum likelihood estimation; tomography; virus structure;
Conference_Titel :
Biomedical Imaging: From Nano to Macro, 2011 IEEE International Symposium on
Conference_Location :
Chicago, IL
Print_ISBN :
978-1-4244-4127-3
Electronic_ISBN :
1945-7928
DOI :
10.1109/ISBI.2011.5872553
