DocumentCode :
3518715
Title :
Autonomous Zebrafish Embryo Injection Using a Microrobotic System
Author :
Wang, W.H. ; Liu, X.Y. ; Sun, Y.
Author_Institution :
Toronto Univ., Toronto
fYear :
2007
fDate :
22-25 Sept. 2007
Firstpage :
363
Lastpage :
368
Abstract :
As an important embodiment of biomanipulation, injection of foreign materials (e.g., DNA, RNAi, sperms, proteins, and drug compounds) into individual cells has significant implications in genetics, transgenics, assisted reproduction, and drug discovery. This paper presents a microrobotic system for fully automated zebrafish embryo injection, which overcomes the problems inherent in manual operation, such as human fatigue and large variations in success rates due to poor reproducibility. Based on computer vision and motion control, the microrobotic system performs injection at a speed of 15 zebrafish embryos (chorion unremoved) per minute, with a survival rate of 98% (350 embryos), a success rate of 99% (350 embryos), and a phenotypic rate of 98.5% (210 embryos). The sample preparation technique and microrobotic control method are applicable to other biological injection applications such as the injection of mouse oocytes/embryos and Drosophila embryos to enable high-throughput biological and pharmaceutical research.
Keywords :
biological specimen preparation; biomedical engineering; medical robotics; micromanipulators; motion control; robot vision; autonomous zebrafish embryo injection; biological injection applications; biomanipulation; computer vision; foreign material cellular injection; microrobotic control method; microrobotic system; motion control; sample preparation technique; Biological materials; DNA; Drugs; Embryo; Fatigue; Genetics; Humans; Manuals; Proteins; Reproducibility of results; Microrobotic cell injection; antisense morpholinos; automation; computer vision; microrobotic control; zebrafish embryos;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Automation Science and Engineering, 2007. CASE 2007. IEEE International Conference on
Conference_Location :
Scottsdale, AZ
Print_ISBN :
978-1-4244-1154-2
Electronic_ISBN :
978-1-4244-1154-2
Type :
conf
DOI :
10.1109/COASE.2007.4341701
Filename :
4341701
Link To Document :
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