Real-time monitoring of photothermal porated mammalian cells by electric impedance sensors

Author

Yamane, Daisuke ; Wu, Yi-Chien ; Wu, Ting-Hsiang ; Toshiyoshi, Hiroshi ; Teitell, Michael A. ; Chiou, Pei-Yu

Author_Institution

Dept. of Mech. & Aerosp. Eng., Univ. of California, Los Angeles, Los Angeles, CA, USA

fYear

2012

fDate

6-9 Aug. 2012

Firstpage

220

Lastpage

221

Abstract

We present a novel electrical impedance sensor for measuring the resealing time of porated mammalian plasma membranes. Openings were generated by high speed cavitation bubbles using laser pulsing of metallic microdisks on transparent ITO electrodes. Real-time impedance measurements show that membrane resealing time and impedance recovery takes 1 to 2 minutes after laser pulsing. Our method can also detect cells under high energy laser pulsing that experience immediate death and a one-way impedance shift.

Keywords

biomembrane transport; bubbles; cavitation; cellular biophysics; electric impedance measurement; electrodes; indium compounds; laser applications in medicine; photothermal effects; InSnO; cavitation bubbles; electric impedance sensors; electrodes; impedance recovery; laser pulsing; membrane resealing time; metallic microdisks; one way impedance shift; photothermal porated mammalian cells; porated mammalian plasma membranes; real time impedance measurements; real time monitoring; Biomembranes; Cells (biology); Impedance; Impedance measurement; Lasers; Measurement by laser beam; Sensors; cell membrane; cell surgery; impedance sensor; membrane pores; photothermal effect; pulsed laser;

fLanguage

English

Publisher

ieee

Conference_Titel

Optical MEMS and Nanophotonics (OMN), 2012 International Conference on

Conference_Location

Banff, AB

ISSN

2160-5033

Print_ISBN

978-1-4577-1511-2

Type

conf

DOI

10.1109/OMEMS.2012.6318882

Filename

6318882