Pneumatically actuated spheroid culturing Lab-on-a-Chip for combinatorial analysis of embryonic body

Author

Nishijima, T. ; Ikeuchi, M. ; Ikuta, K.

Author_Institution

Dept. of Micro-Nano Syst. Eng., Nagoya Univ., Nagoya, Japan

fYear

2012

fDate

Jan. 29 2012-Feb. 2 2012

Firstpage

92

Lastpage

95

Abstract

We have developed conceptually novel Bio-MEMS chip named "PASCL” to break-though the combinational analysis of stem cell differentiation through embryonic body formation. PASCL has the ingenious mechanism for culture and manipulation of EBs by using pneumatically actuated microwells. Each microwell is composed of elastic thin membrane, and its shape can be modified from flat plane to concave microwell by applying pressure to the membrane. By using the active microwells, EBs can be collected from the array selectively and treated with reagents for differentiation inductions at a time. In this paper, spheroid formation and selective collection using PASCL was described for the first time.

Keywords

bioMEMS; biochemistry; bioelectric potentials; biomedical engineering; biomembranes; cellular biophysics; lab-on-a-chip; molecular biophysics; pneumatic actuators; active microwells; bioMEMS chip; combinatorial analysis; elastic thin membrane; embryonic body formation; lab-on-a-chip; pneumatically actuated spheroid; regenerative medicine; spheroid formation; stem cell differentiation; Arrays; Biological systems; Chemicals; Fluids; Pumps; Stem cells; Valves;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on

Conference_Location

Paris

ISSN

1084-6999

Print_ISBN

978-1-4673-0324-8

Type

conf

DOI

10.1109/MEMSYS.2012.6170101

Filename

6170101