DocumentCode
3546681
Title
Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter
Author
Wu, T.-H. ; Chen, Y. ; Park, S.-Y. ; Hong, J. ; Teslaa, T. ; Zhong, J.F. ; Carlo, D. Di ; Teitell, M.A. ; Chiou, P.-Y.
Author_Institution
Univ. of California, Los Angeles (UCLA), Los Angeles, CA, USA
fYear
2012
fDate
Jan. 29 2012-Feb. 2 2012
Firstpage
1097
Lastpage
1100
Abstract
We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20,000 mammalian cells/s with 37% sorting purity, 90% cell viability in enrichment mode, and >;90% purity in high purity mode at 1,500 cells/s or 3,000 beads/s. Fast switching (30 μs) and a small perturbation volume (~90 pL) is realized by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel.
Keywords
bioMEMS; cellular biophysics; fluorescence; high-speed optical techniques; laser beam applications; microfluidics; PDMS channel; PLACS; cell viability; mammalian cells; pulsed laser triggered fluorescence activated cell sorter; sorting purity; Fluorescence; Lasers; Microfluidics; Optical switches; Sorting; Throughput;
fLanguage
English
Publisher
ieee
Conference_Titel
Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on
Conference_Location
Paris
ISSN
1084-6999
Print_ISBN
978-1-4673-0324-8
Type
conf
DOI
10.1109/MEMSYS.2012.6170196
Filename
6170196
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