Towards artificial cell array system: Encapsulation and hydration technologies integrated in liposome array

This work presents two important technologies integrated in our uniform-size liposome array platform (Fig. 1a) to realize an artificial cell array system. One is the effective encapsulation of small objects in the arrayed liposomes (Fig. 1b top), making use of the electrospray deposition technique also used for the lipid patterning. The target nanobeads were selectively patterned on the lipid and encapsulated in the liposomes. Another technology is the liposome formation under biological conditions (Fig. 1b bottom). Thin agarose-gel coating effectively induced the lipid hydration in a saline solution, and also allowed the liposome formation with the in-vitro GFP synthesis solution.