Double-layer hepatocyte tumor co-culture using hydrogel for drug effectivity and specificity analysis

Author

Chin, L.K. ; Luo, K.Q. ; Park, W. ; Je, M. ; Tsai, J. ; Kwong, D.L. ; Liu, A.Q.

Author_Institution

Sch. of Electr. & Electron. Eng., Nanyang Technol. Univ., Singapore, Singapore

fYear

2012

fDate

Jan. 29 2012-Feb. 2 2012

Firstpage

808

Lastpage

811

Abstract

This paper presents the formation of double-layer cellular tumor spheroid for the co-culture of hepatocyte (HepG2 cancer liver cell and LO2 normal liver cell) using alginate hydrogel. With the microfluidic chip, double-layer cellular tumor spheroid can be formed by the hydrogel droplet to mimic the in vivo environment in which the cancer cell is surrounded by normal cell. A microchip with concentration gradient generation and tumor spheroid trapping site is designed for anti-cancer drug analysis.

Keywords

cancer; cellular biophysics; drops; drugs; hydrogels; liver; microfluidics; tumours; HepG2 cancer liver cell; L02 normal liver cell; alginate hydrogel; anticancer drug analysis; double-layer cellular tumor spheroid; double-layer hepatocyte tumor coculture; drug effectivity; hydrogel droplet; microfluidic chip; specificity analysis; tumor spheroid trapping site; Cancer; Charge carrier processes; Drugs; Microfluidics; Minerals; Plugs; Tumors;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems (MEMS), 2012 IEEE 25th International Conference on

Conference_Location

Paris

ISSN

1084-6999

Print_ISBN

978-1-4673-0324-8

Type

conf

DOI

10.1109/MEMSYS.2012.6170309

Filename

6170309