High-throughput white blood cells (leukocytes) separation and enrichment from whole blood by hydrodynamic and inertial force

To solve clogging in microchannel and low separation rate in microfluidic devices that containing high concentration whole blood cells (4.3×10⁶ cells/μl) with the remaining <;1% consisting peripheral blood leukocytes and platelets [1-2]. In this work we introduce a like-biomimetic structure to separate red blood cells from whole blood and to enrich leukocytes (white blood cells) through the elastic properties of blood cell by the interaction of inertial force, shear lift, and wall crowding effect in a continuous flow system. A high separation efficiency (~84%) and high throughput flow rate (200μl/s or 10ml/min) was obtained in this microfluidic chip.