Title :
Analysis of electrode condition for whole cell recording
Author :
Runhuai Yang ; Lai, King W. C. ; Ning Xi ; Jie Yang
Author_Institution :
Univ. of Sci. & Technol. of China, Hefei, China
Abstract :
In this paper, an experimental study on the successful rate of using different types of pipettes for whole cell recording is reported. 5 types of pipettes have been designed and made. The procedure of whole cell recording has been analyzed, and the seal formation of and the rupture of patched membrane have been monitored. According to our research, the optimized value of pipette resistance is 15-30 MΩ for whole cell recording.
Keywords :
bioelectric phenomena; biomechanics; biomedical electrodes; biomembranes; cellular biophysics; fracture; electrode condition analysis; patched membrane rupture monitoring; patched membrane seal formation; pipette resistance; whole cell recording; Biomembranes; Clamps; Extracellular; Immune system; Monitoring; Resistance; Seals;
Conference_Titel :
Nano/Molecular Medicine and Engineering (NANOMED), 2013 IEEE 7th International Conference on
Conference_Location :
Phuket
Print_ISBN :
978-1-4799-2689-3
DOI :
10.1109/NANOMED.2013.6766313
