Title :
Cancer detection using an electronic nose: A preliminary study on detection and discrimination of cancerous cells
Author :
Thriumani, R. ; Zakaria, A. ; Jeffree, A.I. ; Hishamuddin, N.A. ; Omar, M.I. ; Adom, A.H. ; Shakaff, A.Y.M. ; Kamarudin, L.M. ; Yusuf, N. ; Hashim, Y.Z.H.Y. ; Helmy, K.M.
Author_Institution :
Centre of Excellence for Adv. Sensor Technol. (CEASTech), Univ. Malaysia Perlis, Arau, Malaysia
Abstract :
Lack of effective tools to diagnose lung cancer at an early stage has caused high mortality in cancer patients especially in lung cancer patients. Electronic nose (E-Nose) technology is believed to offer non-invasive, rapid and reliable analytic approach by measuring the odour released from cancer to assist medical diagnosis. In this work, using a commercial E-nose (Cyranose-320), we aimed to detect the volatile organic compounds (VOCs) emitted by different types of cancerous cells. The lung cancer cell (A549) and breast cancer cell (MCF-7) were used for this study. Both cells were cultured using Dulbecco´s Modified Eagle´s Medium (DMEM) with 10% of Fetal Bovine Serum (FBS) and incubated for three days. The static headspace of cell cultures and blank medium were directly sniffed by Cyranose-320. The preliminary results from this study showed that, the E-nose is able to detect and distinguish the presence of VOCs in cancerous cells with accuracy of 100% using LDA. To this end, the VOCs emitted from cancerous cells can potentially used as biomarker.
Keywords :
biochemistry; biological tissues; biomedical equipment; cancer; cellular biophysics; electronic noses; lung; molecular biophysics; patient diagnosis; proteins; statistical analysis; A549 cancer cell; Cyranose-320; DMEM; Dulbecco´s modified eagle´s medium; FBS; LDA; MCF-7 cancer cell; VOC detection accuracy; biomarker; breast cancer cell; cancer detection; cancerous cell detection; cancerous cell discrimination; cancerous cell type; cell incubation; commercial e-nose; e-nose technology; early stage lung cancer diagnosis; electronic nose; emitted VOC detection; fetal bovine serum; lung cancer cell; lung cancer diagnosis tool; lung cancer patient mortality; noninvasive analytic approach; preliminary study; rapid analytic approach; released odour measurement; reliable analytic approach; static blank medium headspace; static cell culture headspace; time 3 day; volatile organic compound detection; Cancer; Conferences; Diseases; Electronic noses; Lungs; Principal component analysis; Volatile organic compounds; Cyranose320; Electronic nose; LDA; Lung cancer cell; Volatile organic compounds (VOCs);
Conference_Titel :
Biomedical Engineering and Sciences (IECBES), 2014 IEEE Conference on
DOI :
10.1109/IECBES.2014.7047609
