The effect of tinted lens on full flash electroretinography (ffERG)

The objective of this study was to investigate the effect of different visible light transmission through the tinted lenses on amplitude and implicit time a-wave and b-wave cones adapted full flash electroretinography (ffERG). The percentages of visible light transmission of brown, grey and blue, and blank lens as a control were measured by a spectrophotometer (T80+, PG Instruments) with the software UVWIN 5.0. The ffERG (Mon-Pack One, Metrovision) was used to evaluate the electrophysiology function, which adhered to Clinical Electrophysiology of Vision (ISCEV) standard protocols. Data were analysed using the one-way ANOVA. Sixteen normal eyes from subjects with age range between 18 to 25 years old were recruited via convenience sampling method. There was insignificant difference of a-wave amplitude, (F(3,28) = 0.482, p = 0.697), a-wave implicit time, (F(3,28) = 0.820, p = 0.494), b-wave amplitude, (F(3,28) = 0.075, p = 0.973), and b-wave implicit time, (F(3,28) = 1.264, p = 0.306) towards the effect of four groups of light transmission (blank, brown, grey and blue tinted lenses). Consequently, no difference in ffERG response towards the effect of different light transmission that might be beneficial to the ISCEV protocol, especially to the cataract or IOL person. However, direct testing of electrophysiology function with cataract or IOL simulated is required to confirm.