Scaling up adult stem cells for regenerative medicine

Regenerative medicine is an emerging inter-disciplinary field of research. The ultimate goal for regenerative medicine is to repair or replace damaged, dying cells, tissues or organs. Adult stem cells bioprocessing is one of the options for regenerative medicine albeit the challenges in process development. A few issues on expansion or scaling up media development, handling of cell and bioreactor are reviewed and discussed in this paper. The powerful engineering tools in process characterisation and optimisation using design of experiment (DOE) method is an excellent approach in identifying pertinent features for scaling up stem cells.