Title :
Endothelial cell responses to controlled pulsatile flow at the bifurcation of a model vessel
Author :
Okada, Masahiko ; Sugita, Osamu ; Miida, Takashi ; Makino, Yuko ; Komoro, Noriko
Author_Institution :
Sch. of Med., Niigata Univ., Japan
Abstract :
The authors exposed endothelial cells of the porcine aorta to different flow conditions using a model vessel. This model is made of glass tube and consists of a main tube and a side branch of which angle is 90°. The shear rate of the main tube was 20 sec-1, while that of the side branch was 29 sec-1. Lactic dehydrogenase (LDP) was measured as an indicator of cell damage. Changes of LDH from cultured cells in dishes by adding native lipoprotein, oxidized and non-oxidized linoleic acid were also examined for comparisons. LDH was lower in the cells of the side branch than in those of the main tube
Keywords :
cellular biophysics; haemorheology; pulsatile flow; cell damage indicator; controlled pulsatile flow; endothelial cell responses; flow conditions; glass tube model; lactic dehydrogenase; model vessel bifurcation; native lipoprotein; nonoxidized linoleic acid; oxidized linoleic acid; porcine aorta; Adhesives; Arteries; Atmospheric modeling; Bifurcation; Biochemistry; Cells (biology); Cities and towns; Glass; Laboratories; Muscles; Reservoirs;
Conference_Titel :
Engineering in Medicine and Biology Society, 1994. Engineering Advances: New Opportunities for Biomedical Engineers. Proceedings of the 16th Annual International Conference of the IEEE
Print_ISBN :
0-7803-2050-6
DOI :
10.1109/IEMBS.1994.412039
