Title :
Microwave atomic force microscope: MG63 osteoblast-like cells analysis on nanometer scale
Author :
Lan Zhang ; Yuanhui Song ; Hosoi, Atsushi ; Morita, Yasuyuki ; Yang Ju
Author_Institution :
Dept. of Mech. Sci. & Eng., Nagoya Univ., Nagoya, Japan
Abstract :
In this paper, we report a non-invasive and nondestructive probing method for analyzing the MG63 osteoblast-like cells. High-frequency Microwave atomic force microscope (M-AFM) can sense the topography and microwave image of MG63 cells simultaneously in one scanning process. Under the frequency modulation (FM) AFM mode, this powerful instrument is applied successfully to create a microwave image of MG63 cells with nanometer-scale spatial resolution. By analyzing the results, quantification such as, the number and distribution of organelles and proteins of MG63 cells as well as their dimension and electrical property information can be characterized.
Keywords :
atomic force microscopy; cellular biophysics; medical image processing; microwave imaging; MG63 cell microwave image; MG63 cell topography image; MG63 osteoblast-like cell analysis; electrical property information; frequency modulation; microwave atomic force microscope; nanometer scale; nanometer-scale spatial resolution; nondestructive probing method; Cells (biology); Microscopy; Microwave imaging; Microwave measurement; Microwave theory and techniques; Probes; Proteins; AFM; MG63 cell; Microwave; Non-contact;
Conference_Titel :
Design, Test, Integration and Packaging of MEMS/MOEMS (DTIP), 2014 Symposium on
Print_ISBN :
978-2-35500-028-7
DOI :
10.1109/DTIP.2014.7056644
